Journal: Cellular and Molecular Bioengineering

Article Title: Re-engineering Antimicrobial Peptides into Oncolytics Targeting Drug-Resistant Ovarian Cancers

doi: 10.1007/s12195-020-00626-z

Figure Lengend Snippet: Chemotherapeutic synergy. (a) Isobolograms of MAD1 and Doxorubicin (Dox), Paclitaxel (Ptx) or Cisplatin (Cis) combinatorial synergy in OVCAR-3 (left) and NCI/ADR-RES (right) cells. Fractional inhibitory concentration (FIC) < 1 and < 0.5 represent additive and synergistic effects, respectively. (b) Comparison of Dox and Cis IC50 towards NCI/ADR-RES as either a monotherapy (ADR-RES, black) or in combination with 20 μM MAD1 (ADR-RES + MAD1, grey). Activity of each drug as a monotherapy in pre-resistant OVCAR-3 (OVCAR-3, white) cells shown for comparison. (c–f) Indicated ovarian cancer cell line or patient-derived ovarian carcinoma cells were cultured in ultra-low attachment conditions and treated for 48 h with 2 μM cisplatin or 4 μM MAD1 alone and in combination. Data represent volume in arbitrary units (a.u.) and median.

Article Snippet: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Fmoc-protected amino acids, N,N 0 -diisopropylcarbodiimide (DIC), O-(7-Azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU), N,N -dimethylformamide (DMF), and Paclitaxel were purchased from Chem-Impex International, Inc. RPMI-1640, M199, trypsin and EDTA solution, Antibiotic-Antimycotic Solution, ultra-low attachment (ULA) plates, and 1× phosphate buffered saline (PBS) were purchased from Corning.

Techniques: Concentration Assay, Comparison, Activity Assay, Derivative Assay, Cell Culture

Journal: Foods

Article Title: Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Milk and Other Food Samples

doi: 10.3390/foods11030288

Figure Lengend Snippet: Growth ( a , c ) and phospholipase C reaction ( b , d ) of inclusivity ( n = 110) and exclusivity ( n = 110) test strains. Negative is no-growth, positive is typical growth and atypical is not presumptive Bacillus cereus sensu lato morphology on selective agar media. Abbreviations: PLC—phospholipase C; MYP—mannitol egg yolk polymyxin agar; CH—CHROMagar™ B. cereus ; BA—BACARA ® agar; HI—ChromoSelect Bacillus agar; BRI—Brilliance™ B. cereus agar.

Article Snippet: Within the scope of this study, the performance of commercially available chromogenic selective media ChromoSelect Bacillus (HI; Merck KgaA, Darmstadt, Germany; formerly branded HiCromeTM Bacillus ), CHROMagarTM B. cereus (CH; CHROMagar, Paris, France), BrillianceTM B. cereus (BRI; Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA) and BACARA ® agar (BA; B. cereus Rapid Agar; bioMérieux, Marcy l’Etoile, France) was evaluated in comparison to the ISO recommended standard medium MYP [ ] (Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA).

Techniques:

Journal: Foods

Article Title: Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Milk and Other Food Samples

doi: 10.3390/foods11030288

Figure Lengend Snippet: Typical ( A – E ) and atypical colonies ( F – J ) of Bacillus cereus sensu lato on MYP agar (A (BCG 6), F (BC 66)), ChromoSelect Bacillus agar (B (BC 30), G (BC 20)), Brilliance™ B. cereus agar (C (BC 2), H (BC 19)), CHROMagar™ B. cereus (D (BC 63), I (BC 2)) and BACARA ® agar (E (BC 50), J (BC 34)). Bluish-green colonies are the result of β-D-glucosidase reaction. Precipitation zones surrounding typical colonies are caused by phospholipase C reaction, while lack of mannitol fermentation results in pink background. Abbreviations: MYP—mannitol egg yolk polymyxin agar; HI—ChromoSelect Bacillus agar; BRI—Brilliance™ B. cereus agar; CH—CHROMagar™ Bacillus cereus ; BA—BACARA ® agar.

Article Snippet: Within the scope of this study, the performance of commercially available chromogenic selective media ChromoSelect Bacillus (HI; Merck KgaA, Darmstadt, Germany; formerly branded HiCromeTM Bacillus ), CHROMagarTM B. cereus (CH; CHROMagar, Paris, France), BrillianceTM B. cereus (BRI; Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA) and BACARA ® agar (BA; B. cereus Rapid Agar; bioMérieux, Marcy l’Etoile, France) was evaluated in comparison to the ISO recommended standard medium MYP [ ] (Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA).

Techniques:

Journal: Foods

Article Title: Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Milk and Other Food Samples

doi: 10.3390/foods11030288

Figure Lengend Snippet: Growth of non-target organisms on Bacillus cereus selective media (from upper left to lower right). MYP agar: ( A )— Serratia marcescens (EGN 54); ( B )— Brochothrix thermospacta (EGP 2); ( C )— Bacillus stratosphericus (BG 28). ChromoSelect Bacillus agar: ( D )— Aeromonas hydrophila (EGN 5); ( E )— Acinetobacter baumannii (EGN 2); ( F )— Citrobacter freundii (EGN 9). Brilliance™ B. cereus agar: ( G )— Staphylococcus sciuri (EGP 13); ( H )— Serratia marcescens (EGN 54); ( I )— Pseudomonas fluorescens (EGN 45). CHROMagar™ B. cereus : ( J )— Morganella morganii (EGN 39); ( K )— Enterococcus faecalis (EGP 5); ( L )— Providencia rettgeri (EGN 42). BACARA ® agar: ( M )— Staphylococcus haemolyticus (EGP 11); ( N )— Staphylococcus chromogenes (EGP 9); ( O )– Listeria monocytogenes (EGP 22). Abbreviations: MYP—mannitol egg yolk polymyxin agar; HI—ChromoSelect Bacillus agar; BRI—Brilliance™ B. cereus agar; CH—CHROMagar™ Bacillus cereus ; BA—BACARA ® agar.

Article Snippet: Within the scope of this study, the performance of commercially available chromogenic selective media ChromoSelect Bacillus (HI; Merck KgaA, Darmstadt, Germany; formerly branded HiCromeTM Bacillus ), CHROMagarTM B. cereus (CH; CHROMagar, Paris, France), BrillianceTM B. cereus (BRI; Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA) and BACARA ® agar (BA; B. cereus Rapid Agar; bioMérieux, Marcy l’Etoile, France) was evaluated in comparison to the ISO recommended standard medium MYP [ ] (Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA).

Techniques:

Journal: Foods

Article Title: Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Milk and Other Food Samples

doi: 10.3390/foods11030288

Figure Lengend Snippet: Examples of typical Bacillus cereus sensu lato colonies obtained by sampling of naturally contaminated food ans milk samples. Demarcated colonies with typical morphology (top row): ( A )—MYP agar (ESL-milk); ( B )—HI, ChromoSelect Bacillus agar (ESL-goat milk); ( C )—Brilliance™ B. cereus agar (ESL-milk); ( D )—CHROMagar™ B. cereus (ESL-milk); ( E )—BACARA ® agar (ESL-milk). Colonies masked by high growth of background flora and atypical morphologies (bottom row; arrows point on typical B. cereus colonies): ( F )—coalescing B. cereus colonies surrounded by mannitol-positive background-flora ( B. licheniformis ) on MYP agar (dried fish snack); ( G )—mixed culture on ChromoSelect Bacillus agar, growth of mannitol-positive background-flora ( Staphylococcus spp.) intersparsed with typical colonies (raw milk); ( H )—atypical light colonies with weak β-D-glucosidase activity and typical colonies on Brilliance™ B. cereus agar (ESL-milk); ( I )—atypical PLC-negative and weakly β-D-glucosidase positive colonies lacking the distinctive halo together with typical colony on CHROMagar™ B. cereus (Chinese water spinach); ( J )—atypical small colonies with weak PLC acitivity on BACARA ® agar (dried fish snack). Abbreviations: MYP—mannitol egg yolk polymyxin agar; HI—ChromoSelect Bacillus agar; BRI—Brilliance™ B. cereus agar; CH—CHROMagar™ B. cereus ; BA—BACARA ® agar.

Article Snippet: Within the scope of this study, the performance of commercially available chromogenic selective media ChromoSelect Bacillus (HI; Merck KgaA, Darmstadt, Germany; formerly branded HiCromeTM Bacillus ), CHROMagarTM B. cereus (CH; CHROMagar, Paris, France), BrillianceTM B. cereus (BRI; Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA) and BACARA ® agar (BA; B. cereus Rapid Agar; bioMérieux, Marcy l’Etoile, France) was evaluated in comparison to the ISO recommended standard medium MYP [ ] (Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA).

Techniques: Sampling, Activity Assay

Journal: Foods

Article Title: Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Milk and Other Food Samples

doi: 10.3390/foods11030288

Figure Lengend Snippet: Detectability of Bacillus cereus sensu lato in 64 naturally contaminated food samples before freezing on MYP agar and after freezing on MYP agar and chromogenic media. Abbreviations: MYP—mannitol egg yolk polymyxin agar; BA—BACARA ® agar; CH—CHROMagar™ Bacillus cereus ; BRI—Brilliance™ B. cereus agar; HI—ChromoSelect Bacillus agar.

Article Snippet: Within the scope of this study, the performance of commercially available chromogenic selective media ChromoSelect Bacillus (HI; Merck KgaA, Darmstadt, Germany; formerly branded HiCromeTM Bacillus ), CHROMagarTM B. cereus (CH; CHROMagar, Paris, France), BrillianceTM B. cereus (BRI; Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA) and BACARA ® agar (BA; B. cereus Rapid Agar; bioMérieux, Marcy l’Etoile, France) was evaluated in comparison to the ISO recommended standard medium MYP [ ] (Thermo Fisher Scientific Inc., Oxoid, Waltham, MA, USA).

Techniques: